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KMID : 0624620090420040232
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BMB Reports
2009 Volume.42 No. 4 p.232 ~ p.237
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SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene
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Omura Yasushi
Nishio Yoshihiko
Takemoto Tadashi
Ikeuchi Chikako
Sekine Osamu
Morino Katsutaro
Maeno Yasuhiro
Obata Toshiyuki
Ugi Satoshi
Maegawa Hiroshi
Kimura Hiroshi
Kashiwagi Atsunori
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Abstract
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Sterol regulatory element-binding protein (SREBP)-1c plays a crucial role in the regulation of lipogenic enzymes in the liver. We previously reported that an X-chromosome-linked RNA binding motif (RBMX) regulates the promoter activity of Srebp-1c. However, still unknown was how it regulates the gene expression. To elucidate this mechanism, we screened the cDNA library from mouse liver by yeast two-hybrid assay using RBMX as bait and identified scaffold attachment factor B1 (SAFB1). Immunoprecipitation assay demonstrated binding of SAFB1 to RBMX. Chromatin immunoprecipitation assay showed binding of both SAFB1 and RBMX to the upstream region of Srebp-1c gene. RNA interference of Safb1 reduced the basal and RBMX-induced Srebp-1c promoter activities, resulting in reduced Srebp-1c gene expression. The effect of SAFB1 overexpression on Srebp-1c promoter was found only in the presence of RBMX. These results indicate a major role for SAFB1 in the activation of Srebp-1c through its interaction with RBMX.
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KEYWORD
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Gene regulation, hnRNP, Lipid synthesis, Transcriptional regulation, Yeast two-hybrid assay
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